Human Lysozyme Protein

>100,000 Units/mg protein. 1 unit will produce a change of A450 0.001 per minute at pH-6.24 and 25°C, using a suspension of micrococcus lysodeikticus as substrate in a 2.6ml reaction volume.

Stability: Lyophilized Lysozyme although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Lysozyme Human Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles

Assay Conditions: Recombinant Human Lysozyme is added in lysing bacterial cells to the cell extraction buffer at a range of 0.05-0.5mg/ml (7,000-70,000 U/ml) . The common extraction buffer for E. coli cells contains 0.2mg/ml of recombinant Lysozyme in 100mM Tris-HCl, 2mM EDTA, 0.05% Triton X-100, pH 8. The cell paste is suspended in extraction buffer and incubated for at least 15 min at room temperature. Alternatively, Triton X-100 at 1% and PMSF at 1mM can be added separately and the lysis solution is further incubated for at least 15 min at 37C. In other cases, freeze-thaw cycles, sonication, and mechanical cell disruptions are also used to complement the lysozyme lysis protocol