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Human UCHL3 Protein

Recombinant of human UCHL3 protein. Ubiquitin carboxyl-terminal hydrolase isozyme L3 belongs to a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to produce the ubiquitin monomer. UCHL3 takes part in the regulation of neuronal development and spermatogenesis and is associated to neurodegenerative diseases. UCHL3 has a 54% homology to UCHL1.

Product Specifications

Product Name Alternative

Ubiquitin Carboxyl-Terminal Esterase L3 (ubiquitin thiolesterase), UCH-L3, Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3, EC 3.4.19.12

Tag

20 amino acid His-tag at N-terminus

Source

Escherichia Coli

Concentration

1mg/ml

Purity

> 95% as determined by SDS-PAGE

Bioactivity

Specific activity: >3,000 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37C

Form

Liquid

Molecular Weight

28.3kDa

Storage Conditions

Store at 4°C if the entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) . Avoid freeze thaw cycles.

Notes

For research use only.

Applications Notes

Enzymes

Preservative

20mM Tris-HCl buffer (pH8.0), 1mM DTT, and 10% glycerol.

AA Sequence

MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA

Available Sizes

Frequently Asked Questions

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