UNG enzyme
UNG enzyme
Product Specifications
Product Name Alternative
Uracil DNA Glycosilase, Uracil DNA Glycosylase, UNG.
Source
Escherichia Coli strain that carries the UNG gene from E.coli
Field of Research
Epigenetics & Chromatin
Form
Sterile Filtered colorless solution.
Storage Conditions
Stability: Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored below -18°C. Please prevent freeze-thaw cycles
Notes
For research use only.
Applications Notes
Unit Definition: 1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37°C. Inactivation: Inactivated by heating at 95°C for 10min. Enzyme activity is partially restored at temperatures lower than 55°C. Reaction Conditions: 1X UNG Reaction Buffer, incubate at 37°C.UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM) . The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites. 10X UNG Reaction Buffer: 200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA
Preservative
UNG solution in 10mM Tris-HCl (pH-7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml rAlbumin and 50% glycerol.
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