UNG enzyme

Unit Definition: 1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37°C. Inactivation: Inactivated by heating at 95°C for 10min. Enzyme activity is partially restored at temperatures lower than 55°C. Reaction Conditions: 1X UNG Reaction Buffer, incubate at 37°C.UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM) . The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites. 10X UNG Reaction Buffer: 200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA