What is the catalog number for UNG enzyme?

The catalog number for UNG enzyme is orb423166.

What sizes are available for UNG enzyme?

UNG enzyme is available in the following size(s): 2000 U, 10000 U, 20000 U.

How many pack sizes does UNG enzyme come in?

UNG enzyme is available in 3 different pack sizes.

Is UNG enzyme in stock?

UNG enzyme is currently made to order / not held in immediate stock. Contact us for lead time.

UNG enzyme

Product Specifications

Product Name Alternative

Uracil DNA Glycosilase, Uracil DNA Glycosylase, UNG.

Source

Escherichia Coli strain that carries the UNG gene from E.coli

Field of Research

Epigenetics & Chromatin

Form

Sterile Filtered colorless solution.

Storage Conditions

Stability: Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored below -18°C. Please prevent freeze-thaw cycles

Notes

For research use only.

Applications Notes

Unit Definition: 1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37°C. Inactivation: Inactivated by heating at 95°C for 10min. Enzyme activity is partially restored at temperatures lower than 55°C. Reaction Conditions: 1X UNG Reaction Buffer, incubate at 37°C.UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM) . The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites. 10X UNG Reaction Buffer: 200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA