Human C-JUN Protein
Recombinant of Human C-JUN protein
Product Specifications
Product Name Alternative
Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, c-Jun.
Source
Escherichia Coli
Field of Research
Cancer Biology
Purity
Greater than 95% as determined by SDS-PAGE.
Bioactivity
C-Jun is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with JNK immunoprecipitated from stimulated cells. This phosphorylation can be monitored by Western blot analysis using an antibody directed to c-Jun [pS73], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to centrifuge the vial prior to opening in order to bring the contents to the bottom. The reconstitution of the lyophilized c-Jun is recommended in 40mM Tris, pH 7.5, to a concentration of 0.2-1.0 mg/ml.
Storage Conditions
Stability: Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles
Notes
For research use only.
Applications Notes
Note: Kinase activity may vary depending on the substrate and reaction conditions
Preservative
C-JUN is supplied as lyophilized powder containing no additives.
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
ICP158 00125 125 mL
Praseodymium (Pr) ICP Standard Solution 1.0 gm/L In 5% HNO3 Traceable To NIST -1000 ppm 5% v/v Nitric Acid
Sign In for Pricing
View Details