Human Bcl 2 (minus NWGR domain) Protein
Recombinant of human Bcl 2 (minus NWGR domain) protein
Product Specifications
Product Name Alternative
Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2.
Source
Escherichia Coli
Purity
Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
Suspend Bcl-2 in 100 μl of 0.5M Acetic acid, over night at 4°C. Dilute 10 fold into selected buffer system.BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.
Storage Conditions
Stability: Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles
Notes
For research use only.
Applications Notes
Applications: Input marker or positive control (Western Blotting) .Function study
Preservative
The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
IRBAHURAB4AACF18C100UL 100 µL
Rabbit Anti Human RAB4A Monoclonal Clone ACF-18 100UL
Sign In for Pricing
View Details 