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Human TNF a Protein (His Tag)

Recombinant of Human TNF a protein (His Tag)

Product Specifications

Product Name Alternative

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Source

Escherichia Coli

Field of Research

Immunology & Inflammation

Purity

Greater than 97.0% as determined by SDS-PAGE and HPLC analyses.

Bioactivity

The ED50 was determined in the presence of actinomycin D by a cytotoxicity assay using murine L929 cells is 2.0 x 107IU/mg.

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Solubility

It is recommended to reconstitute the lyophilized TNF-α in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions

Stability: Lyophilized TNF-α although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-α should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles

Notes

For research use only.

Applications Notes

Cytokines And Growth Factors

Preservative

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.

AA Sequence

MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL

Available Sizes

Frequently Asked Questions

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