Human Bcl 2 (minus BH3 domain) Protein
Recombinant of Human Bcl 2 (minus BH3 domain) protein
Product Specifications
Product Name Alternative
Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2.
Source
Escherichia Coli
Purity
Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
Suspend Bcl-2 in 100 μl of 0.5M Acetic acid, over night at 4°C. Dilute 10 fold into selected buffer system.BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.
Storage Conditions
Stability: Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles
Notes
For research use only.
Applications Notes
Applications: Input marker or positive control (Western Blotting) .Function study
Preservative
The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.
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