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Human Collagen IV Protein

Recombinant Human Collagen IV protein

Product Specifications

Product Name Alternative

Collagen alpha-3 (IV) chain, Goodpasture antigen, COL4A3, Glomerular Basal Membrane, GBM.

Source

Sf9 insect cells

Field of Research

Musculoskeletal & Connective Tissue Research

Purity

Greater than 87% as determined by Capillary Electrophoresis.

Form

Sterile Filtered clear solution.

Storage Conditions

Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles

Notes

For research use only.

Applications Notes

Note: It has been reported that the immunodominant epitope of COL4A3 is a cryptic epitope that is not easily accessible to the corresponding autoantibodies. It is necessary to treat the protein under nonreducing conditions with a denaturant such as urea to unmask the epitopes (see Hellmark et al. in Autoantibodies, Peter, J.B. and Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298)

Preservative

COL4A3 Human biotin contains 20mM HEPES, pH 7.5, 0.02% SDS and 0.01mM EDTA.

Available Sizes

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