Cys-Protein-G Protein
Cys-Protein G is produced in E. coli as a single, non-glycosylated polypeptide consisting of 201 amino acids with an N-terminal cysteine residue. It has a predicted molecular weight of approximately 21.9 kDa, but migrates with an apparent molecular weight of about 40 kDa on SDS-PAGE.
Product Specifications
Source
Escherichia Coli
Field of Research
Signal Transduction
Purity
Greater than 95.0% as determined by SDS-PAGE and HPLC analyses.
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized Protein G in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Storage Conditions
Stability: Lyophilized Protein G although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Protein G should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles
Notes
For research use only.
Applications Notes
Recombinant & Natural Proteins
Preservative
Lyophilized without any additives.
AA Sequence
CLPKTDTYKL ILNGKTLKGE TTTEAVDAAT AEKVFKQYAN DNGVDGEWTY DDATKTFTVT EKPEVIDASE LTPAVTTYKL VINGKTLKGE TTTEAVDAAT AEKVFKQYAN DNGVDGEWTY DDATKTFTVT EKPEVIDASE LTPAVTTYKL VINGKTLKGE TTTKAVDAET AEKAFKQYAN DNGVDGVWTY DDATKTFTVT E
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