Dihydrofluorescein diacetate

Dihydrofluorescein diacetate is a fluorimetric probe mainly used for oxidative stress measurements, in both cell-free systems and cellular models.

Product Specifications

CAS Number

[35340-49-9]

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/Dihydrofluorescein_diacetate.html

Purity

98.48

Solubility

DMSO : ≥ 106.5 mg/mL

Smiles

O=C(O)C1=CC=CC=C1C2C3=C(OC4=C2C=CC(OC(C)=O)=C4)C=C(OC(C)=O)C=C3

Molecular Formula

C24H18O7

Molecular Weight

418.40

Precautions

H302, H315, H319, H335

References & Citations

[1]Hempel SL, et al. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5 (and 6) -carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med. 1999 Jul;27 (1-2) :146-59.|[2]Diaz G, et al. Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes. Histochem Cell Biol. 2003 Oct;120 (4) :319-25.|[3]Aranda A, et al. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment of nanoparticle-treated cells. Toxicol In Vitro. 2013 Mar;27 (2) :954-63.