Denhardt's Solution 100X (Biotechnology Grade)

<strong>Denhardt's Solution 100X (Biotechnology Grade)</strong>_x000D_ <table width="1028">_x000D_ <tbody>_x000D_ <tr>_x000D_ <td width="285">Catalog number:</td>_x000D_ <td width="743">B2010088</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Lot number:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Expiration date:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Volume/Weight</td>_x000D_ <td>50 mL</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>pH</td>_x000D_ <td>N/A</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Supplied as</td>_x000D_ <td>100X concentrate</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Appearance</td>_x000D_ <td>Clear solution</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Applications</td>_x000D_ <td>Denhardt's solution is highly reproducible, pure and filter-sterilized solution suitable for nucleic acid hybridization. This solution consists of a mixture of special polymers capable of suppressing non-specific binding sites and increasing the concentration of nucleic acid probes.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Shelf-Life</td>_x000D_ <td>1 year from date of manufacture</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Storage:</td>_x000D_ <td>Keep at 2-8°C</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Keywords:</td>_x000D_ <td>denhardt's in situ hybridization, denhardt solution, denhardt's buffer, denhardt buffer</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Grade</td>_x000D_ <td>Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered on a 0.22 um.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>References</td>_x000D_ <td width="743">1: Goldau T, Murayama K, Brieke C, Asanuma H, Heckel A. Azobenzene C-Nucleosides_x000D_ for Photocontrolled Hybridization of DNA at Room Temperature. Chemistry. 2015_x000D_ Dec 1;21(49):17870-6.2: Markegard CB, Gallivan CP, Cheng DD, Nguyen HD. Effects of Concentration and_x000D_ Temperature on DNA Hybridization by Two Closely Related Sequences via Large-_x000D_ Scale Coarse-Grained Simulations. J Phys Chem B. 2016 Aug 18;120(32):7795-806._x000D_ _x000D_ 3: do Nascimento C, Muller K, Sato S, Albuquerque Junior RF. Effect of sample_x000D_ storage time on detection of hybridization signals in Checkerboard DNA-DNA_x000D_ hybridization. Can J Microbiol. 2012 Apr;58(4):502-6._x000D_ 4: Avarre JC, de Lajudie P, Béna G. Hybridization of genomic DNA to microarrays:_x000D_ a challenge for the analysis of environmental samples. J Microbiol Methods. 2007_x000D_ May;69(2):242-8._x000D_ 5: Cho JC, Tiedje JM. Bacterial species determination from DNA-DNA hybridization_x000D_ by using genome fragments and DNA microarrays. Appl Environ Microbiol. 2001_x000D_ Aug;67(8):3677-82._x000D_ 6: Su X, Wu YJ, Knoll W. Comparison of surface plasmon resonance spectroscopy_x000D_ and quartz crystal microbalance techniques for studying DNA assembly and_x000D_ hybridization. Biosens Bioelectron. 2005 Nov 15;21(5):719-26._x000D_ _x000D_ 7: Hu Y, Yang T, Li Q, Guan Q, Jiao K. Conjugated self-doped polyaniline-DNA_x000D_ hybrid as trigger for highly sensitive reagentless and electrochemical self-_x000D_ signal amplifying DNA hybridization sensing. <a href="https://pubmed.ncbi.nlm.nih.gov/23304697/">Analyst</a>. 2013 Feb_x000D_ 21;138(4):1067-74._x000D_ _x000D_ 8: Durnam DM, Anders KR, Fisher L, O'Quigley J, Bryant EM, Thomas ED. Analysis_x000D_ of the origin of marrow cells in bone marrow transplant recipients using a_x000D_ Y-chromosome-specific in situ hybridization assay. Blood. 1989 Nov_x000D_ 1;74(6):2220-6. PMID: 2804360.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Related Products</td>_x000D_ <td><a href="https://moleculardepot.com/product-category/buffers/">Biochemical Buffers and Solutions</a></td>_x000D_ </tr>_x000D_ </tbody>_x000D_ </table>