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TEV Protein

Tobacco Etch Virus Protease Recombinant protein. TEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the tobacco etch virus (TEV) . Because its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins. TEV protease recognizes a linear epitope of the general form E-Xaa-Xaa-Y -Xaa-Q- (G/S), with cleavage occurring between Q and G or Q and S. The most commonly used sequence is ENLYFQG.

Product Specifications

Product Name Alternative

RTEV, TEV, P1 protease.

Source

Escherichia Coli

Purity

Greater than 90.0% as determined by analysis by SDS-PAGE.

Form

Sterile liquid formulation.

Storage Conditions

Stability: rTEV although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles

Notes

For research use only.

Applications Notes

Unit Definition: One unit is defined as the amount of enzyme needed to cleave 3 ug of fusion protein in 1 hour to 85 % completion at 30°C in a buffer containing 50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, and 1 mM DTT. Cleavage Conditions: A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat-labile, then 4°C incubations are recommended. Reactions at 4°C will require longer incubation times and/or more rTEV

Preservative

The rTEV contains 25mM Tris, pH 8.0, 75mM NaCl, 5mM EDTA, 10mM GSH, 50% Glycerol.

Available Sizes

Frequently Asked Questions

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