Npc1 (mouse) Antibody
Goat polyclonal antibody to NPC1
Product Specifications
Product Name Alternative
Anti-Niemann Pick C1 protein precursor antibody, anti-Niemann Pick disease, type C1 antibody, anti-Niemann-Pick C1 protein antibody, anti-NPC antibody, anti-NPC1 antibody, anti-NPC-1 antibody, anti-NPC 1 antibody
Target
Npc1 (mouse)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
TYHTILKTSADYTD
Field of Research
Epigenetics, Infectious Diseases
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 1 μg/ml
Molecular Weight
142
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 36kDa band in lysates of cell lines NIH3T3 and NSO after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 142kDa according to NP_032746.2. The 36kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Mouse
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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