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Npc1 (mouse) Antibody

Goat polyclonal antibody to NPC1

Product Specifications

Product Name Alternative

Anti-Niemann Pick C1 protein precursor antibody, anti-Niemann Pick disease, type C1 antibody, anti-Niemann-Pick C1 protein antibody, anti-NPC antibody, anti-NPC1 antibody, anti-NPC-1 antibody, anti-NPC 1 antibody

Target

Npc1 (mouse)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

TYHTILKTSADYTD

Field of Research

Epigenetics, Infectious Diseases

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 1 μg/ml

Molecular Weight

142

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 36kDa band in lysates of cell lines NIH3T3 and NSO after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 142kDa according to NP_032746.2. The 36kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Mouse

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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