Recombinant Bacillus thuringiensis Pesticidal crystal protein cry1Bb (cry1Bb), partial

Product Specifications

Product Name Alternative

(140 kDa crystal protein) (Crystaline entomocidal protoxin) (Insecticidal delta-endotoxin CryIB (b) )

Abbreviation

Recombinant Bacillus thuringiensis cry1Bb protein, partial

Gene Name

Cry1Bb

UniProt

Q45739

Expression Region

1111-1229aa

Organism

Bacillus thuringiensis

Target Sequence

NCEEEEVYPTDTGTCNDYTAHQGTAACNSRNAGYEDAYEVDTTASVNYKPTYEEETYTDVRRDNHCEYDRGYVNYPPVPAGYVTKELEYFPETDTVWIEIGETEGKFIVDSVELLLMEE

Tag

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Others

Relevance

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

21.1 kDa

References & Citations

"Bacillus thuringiensis cryet4 and cryet5 toxin genes and proteins toxic to lepidopteran insects." Donovan W.P., Tan Y., Jany C.S., Gonzalez J.M. Jr. Patent number US5322687, 21-JUN-1994

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.