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RDH5 Antibody

Goat polyclonal antibody to RDH5

Product Specifications

Product Name Alternative

Anti 11-cis RDH antibody, anti 9-cis-retinol specific dehydrogenase antibody, anti FLJ39337 antibody, anti FLJ97089 antibody, anti HSD17B9 antibody, anti RDH1 antibody, anti retinol dehydrogenase 1 antibody, anti retinol dehydrogenase 5 (11-cis and 9-cis) antibody, anti retinol dehydrogenase 5 (11-cis/9-cis) antibody, anti SDR9C5 antibody, anti short chain dehydrogenase/reductase family 9C, antibody, anti RDH5 antibody

Target

RDH5

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

RTPVTNLESLEKT

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 0.3 μg/ml

Molecular Weight

35

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave bands at approx 70kDa and 40kDa in Mouse Eye lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 35.0kDa according to NP_002896.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Human, Rat

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

5959

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