POU5F1B Antibody

Goat polyclonal antibody to POU5F1B

Product Specifications

Product Name Alternative

Anti OCT4-pg1 antibody, anti octamer b antibody, anti OTF3C antibody, anti OTF3P1 antibody, anti POU 5 domain protein antibody, anti POU class 5 homeobox 1 pseudogene 1 antibody, anti POU class 5 homeobox 1B antibody, anti POU domain transcription factor Oct-4 antibody, anti POU domain, class 5, transcription factor 1 pseudogene 1 antibody, anti POU5F1P1 antibody, anti POU5FLC20 antibody, anti POU5FLC8 antibody, anti POU5F1B antibody

Target

POU5F1B

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

TPGYGSPHFTALYS

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 1 μg/ml

Molecular Weight

38.6

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.6kDa according to NP_001153014.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Human

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.