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LARGE (N-terminus) Antibody

Goat polyclonal antibody to LARGE

Product Specifications

Product Name Alternative

Anti acetylglucosaminyltransferase-like 1A antibody, anti acetylglucosaminyltransferase-like protein antibody, anti KIAA0609 antibody, anti like-acetylglucosaminyltransferase antibody, anti like-glycosyltransferase antibody, anti MDC1D antibody, anti LARGE antibody

Target

LARGE (N-terminus)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

RYTASSQRERESLE

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.5 μg/ml

Molecular Weight

88.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 125kDa band in Human Kidney, Pancreas and Placenta lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 88.1kDa according to NP_004728.1. The 125kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Human, Mouse, Rat

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

9215

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