LARGE (N-terminus) Antibody
Goat polyclonal antibody to LARGE
Product Specifications
Product Name Alternative
Anti acetylglucosaminyltransferase-like 1A antibody, anti acetylglucosaminyltransferase-like protein antibody, anti KIAA0609 antibody, anti like-acetylglucosaminyltransferase antibody, anti like-glycosyltransferase antibody, anti MDC1D antibody, anti LARGE antibody
Target
LARGE (N-terminus)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
RYTASSQRERESLE
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.5 μg/ml
Molecular Weight
88.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 125kDa band in Human Kidney, Pancreas and Placenta lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 88.1kDa according to NP_004728.1. The 125kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Human, Mouse, Rat
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
9215
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