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TRIM71 (aa409-419) Antibody

Goat polyclonal antibody to TRIM71

Product Specifications

Product Name Alternative

Anti abnormal cell LINeage LIN-41 antibody, anti homolog of C. elegans Lin-41 antibody, anti LIN41 antibody, anti LIN-41 antibody, anti tripartite motif-containing 71 antibody, anti TRIM71 antibody

Reactivity

Mouse

Target

TRIM71 (aa409-419)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

NKLESTISAVQ

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 1-3 μg/ml

Molecular Weight

93.4

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 100kDa band observed in lysates of cell line NIH3T3 (calculated MW of 93.4kDa according to Human NP_001034200.1 and of 92.1kDa according to Mouse NP_001035968.1) . Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 14kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Canine, Human, Rat

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

131405

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