TRIM71 (aa409-419) Antibody
Goat polyclonal antibody to TRIM71
Product Specifications
Product Name Alternative
Anti abnormal cell LINeage LIN-41 antibody, anti homolog of C. elegans Lin-41 antibody, anti LIN41 antibody, anti LIN-41 antibody, anti tripartite motif-containing 71 antibody, anti TRIM71 antibody
Reactivity
Mouse
Target
TRIM71 (aa409-419)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
NKLESTISAVQ
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 1-3 μg/ml
Molecular Weight
93.4
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 100kDa band observed in lysates of cell line NIH3T3 (calculated MW of 93.4kDa according to Human NP_001034200.1 and of 92.1kDa according to Mouse NP_001035968.1) . Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 14kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Canine, Human, Rat
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
131405
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