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Goat anti-Cryopirin / NALP3 / NLRP3 Antibody

Selected Variant

GTR17698144

Size: 100 µg

Goat polyclonal antibody to Cryopyrin

Product Specifications

Product Name Alternative

Anti AGTAVPRL antibody, anti C1orf7 antibody, anti Caterpiller protein 1.1 antibody, anti CIAS 1 antibody, anti CIAS1 antibody, anti FCAS antibody, anti FCU antibody, anti MWS antibody, anti NACHT antibody, anti NALP3 antibody, anti NALP 3 antibody, anti NALP-3 antibody, anti NLRP3 antibody, anti PYPAF 1 antibody, anti PYPAF1 antibody, anti PYRIN containing APAF1 like protein 1 antibody

Reactivity

Human

Target

Cryopirin / NALP3 / NLRP3

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

RDLYEKAKRDEPK

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml

Molecular Weight

118; 106; 112; 112

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 36kDa band in Human Peripheral Blood Mononucleocytes lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the smallest calculated size of 106kDa according to NP_899632.1. The 36kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

114548

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