Goat anti-Cryopirin / NALP3 / NLRP3 Antibody
Selected Variant
GTR17698144
Size: 100 µg
Goat polyclonal antibody to Cryopyrin
Product Specifications
Product Name Alternative
Anti AGTAVPRL antibody, anti C1orf7 antibody, anti Caterpiller protein 1.1 antibody, anti CIAS 1 antibody, anti CIAS1 antibody, anti FCAS antibody, anti FCU antibody, anti MWS antibody, anti NACHT antibody, anti NALP3 antibody, anti NALP 3 antibody, anti NALP-3 antibody, anti NLRP3 antibody, anti PYPAF 1 antibody, anti PYPAF1 antibody, anti PYRIN containing APAF1 like protein 1 antibody
Reactivity
Human
Target
Cryopirin / NALP3 / NLRP3
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
RDLYEKAKRDEPK
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
Molecular Weight
118; 106; 112; 112
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 36kDa band in Human Peripheral Blood Mononucleocytes lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the smallest calculated size of 106kDa according to NP_899632.1. The 36kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
114548
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