Human AKT3 protein

Recombinant protein corresponds to amino acids 1 to 481 of mature human AKT3; Akt isoform 3. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. This protein was co-expressed with the p110 kinase domain leading to phosphorylation of key residues T308 and S473. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.

Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Human AKT3 recombinant protein has been tested in western blot and SDS-Page and is suitable as a control for polyclonal or monoclonal anti-AKT3 in immunological assays. Akt3 recombinant protein is expected to be phosphorylated and in an active state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized