Llama IgG Whole Molecule Antibody

Llama IgG has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. This product contains equal amounts of IgG1, IgG2 and IgG3. Coomassie stained SDS-PAGE of non-reduced llama IgG1 shows a band of ~150 kDa whereas the reduced form exhibits ~55 kDa (heavy chain) and ~25 kDa (light chain) ; non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa; non-reduced llama IgG3 shows a band of ~100 kDa whereas the reduced form exhibits ~43 kDa.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Llama IgG whole molecule has been tested by SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, flow cytometry, and ELISA experiments. Specific conditions for reactivity should be optimized by the end user. Llama IgG whole molecule buffered in 0.015 M Potassium Phosphate, 0.01125 M Sodium Chloride, pH 7.2 and 25% glycerol.