Swine IgM Antibody
Swine IgM Antibody
Product Specifications
Product Name Alternative
Swine immunoglobulin M, Pig IgM
Conjugation
Unconjugated
Source
Swine
Origin
Swine
Field of Research
Protein Biochemistry
Concentration
1.0 mg/mL
Dilution
ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Purity
Swine IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Swine IgM whole molecule was assayed by immunoelectrophoresis resulting in a single precipitin arc against anti-Swine Serum and anti-Swine IgM (μ chain specific) . No reaction was observed against anti-Swine IgG F (c) . Some light chain cross reactivity will occur with anti-Swine IgG.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. Swine IgM whole molecule is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Swine IgM whole molecule has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Tested Applications
SDS-PAGE
Preservative
Preservative: 0.1% (w/v) Sodium Azide; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
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