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Swine IgM Antibody

Swine IgM Antibody

Product Specifications

Product Name Alternative

Swine immunoglobulin M, Pig IgM

Conjugation

Unconjugated

Source

Swine

Origin

Swine

Field of Research

Protein Biochemistry

Concentration

1.0 mg/mL

Dilution

ELISA: User Optimized, IHC: User Optimized, WB: User Optimized

Purity

Swine IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Swine IgM whole molecule was assayed by immunoelectrophoresis resulting in a single precipitin arc against anti-Swine Serum and anti-Swine IgM (μ chain specific) . No reaction was observed against anti-Swine IgG F (c) . Some light chain cross reactivity will occur with anti-Swine IgG.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. Swine IgM whole molecule is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Swine IgM whole molecule has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Tested Applications

SDS-PAGE

Preservative

Preservative: 0.1% (w/v) Sodium Azide; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

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