Rat IgM Antibody
Rat IgM Antibody
Product Specifications
Product Name Alternative
Rat Immunoglobulin M
Conjugation
Unconjugated
Source
Rat
Origin
Rat
Field of Research
Protein Biochemistry
Concentration
1.1 mg/mL
Dilution
ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Purity
Rat IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Rat IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum and anti-Rat IgM (μ chain specific) . No reaction was observed against anti-Rat IgG F (c) . Some light chain cross-reactivity will occur with anti-Rat IgG.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. Rat IgM whole molecule is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Rat IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Tested Applications
SDS-PAGE
Preservative
Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
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