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Rat IgM Antibody

Rat IgM Antibody

Product Specifications

Product Name Alternative

Rat Immunoglobulin M

Conjugation

Unconjugated

Source

Rat

Origin

Rat

Field of Research

Protein Biochemistry

Concentration

1.1 mg/mL

Dilution

ELISA: User Optimized, IHC: User Optimized, WB: User Optimized

Purity

Rat IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Rat IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum and anti-Rat IgM (μ chain specific) . No reaction was observed against anti-Rat IgG F (c) . Some light chain cross-reactivity will occur with anti-Rat IgG.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. Rat IgM whole molecule is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Rat IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Tested Applications

SDS-PAGE

Preservative

Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

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