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Rabbit IgG F (ab') 2 Antibody

Rabbit IgG F (ab') 2 Antibody

Product Specifications

Product Name Alternative

Rabbit Immunoglobulin IgG F (ab') 2 fragment, IgG Fab2

Conjugation

Unconjugated

Source

Rabbit

Origin

Rabbit

Field of Research

Protein Biochemistry

Concentration

2 mg/mL

Dilution

ELISA: User Optimized, WB: User Optimized

Purity

Rabbit IgG F (ab') 2 was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG, anti-Rabbit IgG F (ab’) 2 and anti-Rabbit Serum. No reaction was observed against anti-Rabbit IgG F (c) or anti-Pepsin.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Rabbit IgG F (ab') 2 Fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

Tested Applications

SDS-PAGE

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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