Mouse IgG F (ab') 2 Antibody

Mouse IgG F (ab') 2 fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Mouse IgG and anti-Mouse IgG F (ab’) 2. No reaction was observed against anti-Mouse IgG F (c) or anti-Pepsin.

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Mouse IgG F (ab') 2 fragment is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Mouse IgG F (ab') 2 fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.