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Mouse IgG F (c) Antibody

Mouse IgG F (c) Antibody

Product Specifications

Product Name Alternative

MOUSE IgG F (c) fragment, Mouse Fc, Immunoglobulin Fc

Conjugation

Unconjugated

Source

Mouse

Origin

Mouse

Field of Research

Protein Biochemistry

Concentration

1.1 mg/mL

Dilution

ELISA: User Optimized, IHC: User Optimized, WB: User Optimized

Purity

MOUSE IgG F (c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Mouse IgG and anti-Mouse IgG F (c) . No reaction was observed against anti-Mouse IgG F (ab’) 2 or anti-Papain.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Mouse IgG Fc fragment is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Mouse IgG F (c) Fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Tested Applications

SDS-PAGE

Preservative

Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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