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Human Transferrin protein

Human Transferrin protein

Product Specifications

Product Name Alternative

Apotransferrin, Transferrin Control, Siderophilin, TRF, apo-hTF

UniProt

Q06AH7

Conjugation

Unconjugated

Source

Human

Origin

Human

Field of Research

Protein Biochemistry

Concentration

10.0 mg/ml

Dilution

ELISA: 1:10,000 - 1:20,000, WB: 1:2,000-1:10,000

Purity

Human transferrin was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation followed by extensive dialysis. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Transferrin and anti-Human Serum.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Human Apo Transferrin from human serum was lyophilized from 0.2M ammonium bicarbonate for use in cell culture and assays. Transferrin has been tested in SDS-Page and is also suitable for use as antigen or ligand in immunochemical reactions, as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified proteins.

Tested Applications

ELISA, SDS-PAGE, WB

NCBI Accession Number

AAB22049.1

Preservative

Preservative: None. Stabilizer: None; Buffer: See application note.

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