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Human IgG F (ab') 2 Antibody

Human IgG F (ab') 2 Antibody

Product Specifications

Product Name Alternative

Human Immunoglobulin G F (ab') 2 Fragment, IgG Fab2

Conjugation

Unconjugated

Source

Human

Origin

Human

Field of Research

Protein Biochemistry

Concentration

2.0 mg/mL

Dilution

ELISA: 1:5000 - 1:50,000, WB: 1:1000

Purity

Human IgG F (ab') 2 was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F (ab’) 2. No reaction was observed against anti-Human IgG F (c) or anti-Pepsin.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Human IgG F (ab') 2 Fragment has been tested in SDS-Page and ELISA and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

Tested Applications

ELISA, SDS-PAGE, WB

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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