Human IgG F (ab') 2 Antibody
Human IgG F (ab') 2 Antibody
Product Specifications
Product Name Alternative
Human Immunoglobulin G F (ab') 2 Fragment, IgG Fab2
Conjugation
Unconjugated
Source
Human
Origin
Human
Field of Research
Protein Biochemistry
Concentration
2.0 mg/mL
Dilution
ELISA: 1:5000 - 1:50,000, WB: 1:1000
Purity
Human IgG F (ab') 2 was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F (ab’) 2. No reaction was observed against anti-Human IgG F (c) or anti-Pepsin.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Human IgG F (ab') 2 Fragment has been tested in SDS-Page and ELISA and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.
Tested Applications
ELISA, SDS-PAGE, WB
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog