Human IgG Antibody
Human IgG Antibody
Product Specifications
Product Name Alternative
Human IgG whole molecule, Human Immunoglobulin G
Conjugation
Unconjugated
Source
Human
Origin
Human
Field of Research
Protein Biochemistry
Concentration
10.846 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, FC: 1:500, WB: 1:1,000 - 1:5,000
Purity
IgG was prepared from normal human serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human IgG and anti-Human Serum.
Form
Lyophilized
Storage Conditions
Store Human IgG at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Human IgG whole molecule has been tested by SDS-Page and ELISA and can be utilized as a control or standard reagent in Western Blotting, Flow, and ELISA experiments.
Tested Applications
ELISA, FC, SDS-PAGE, WB
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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