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Human IgG PE Antibody

Human IgG PE Antibody

Product Specifications

Product Name Alternative

Human IgG Phycoerythrin conjugated, Human IgG PE conjugated, isotype control for flow cytometry

Conjugation

RPE

Source

Human

Origin

Human

Field of Research

Protein Biochemistry

Concentration

0.5 mg/mL

Dilution

FC: 1:500-1:2500, IHC: 1:500-1:2500, WB: 1:1,000-1:5,000

Purity

Human IgG whole molecule Phycoerythrin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above. Human IgG whole molecule Phycoerythrin conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Human IgG and anti-Human Serum.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent) . This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Notes

For research use only.

Applications Notes

Human IgG whole molecule Phycoerythrin conjugated can be utilized as a control reagent in Flow Cytometry, Immunohistochemistry, and Western Blotting. Researchers should determine optimal titers for applications that are not stated.

Tested Applications

FC, IHC, WB

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01M Magnesium Chloride and 0.01M Beta-Mercaptoethanol

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