Chicken IgG Fab Biotin Antibody

This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Chicken IgG, anti-Chicken IgG F (ab’) 2 and anti-Chicken Serum. No reaction was observed against anti-Chicken IgG F (c) or anti-Papain.

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Chicken IgG F (ab) Biotin Conjugated has been tested by SDS-PAGE and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2