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DbpB Control protein

DbpB Control protein

Product Specifications

Product Name Alternative

Decorin-binding protein B, Borrelia burgdorferi DbpB, control protein, DbpB Control Protein

UniProt

P0CL68

Conjugation

Unconjugated

Source

Borrelia burgdorferi

Origin

Borrelia burgdorferi

Field of Research

Signal Transduction

Concentration

1.0 mg/mL

Dilution

ELISA: User Optimized, WB: User Optimized

Purity

DbpB is a fusion protein synthesized with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified DbpB and was estimated to be greater than 90% pure.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

DbpB protein has been tested by SDS-PAGE and is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect bands at 60.3 kDa for DbpB-MBP, (17.9 kDa for DbpB and 42.4 kDa for MBP) in size corresponding to DbpB by Western blotting in the appropriate cell lysate or extract.

Tested Applications

SDS-PAGE, WB

NCBI Accession Number

WP_010890381.1

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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