OspB Control protein
OspB Control protein
Product Specifications
Product Name Alternative
Outer surface protein B, Borrelia burgdorferi OspB, control protein
UniProt
P17739
Conjugation
Unconjugated
Source
Borrelia burgdorferi
Origin
Borrelia burgdorferi
Field of Research
Protein Biochemistry
Concentration
1 mg/mL
Dilution
ELISA: User Optimized, WB: User Optimized
Purity
OspB is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified OspB and was estimated to be greater than 90% pure.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
OspB is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 72.7 kDa for OspB-MBP, (30.3 kDa for OspB and 42.4 for MBP) in size corresponding to OspB by Western blotting in the appropriate cell lysate or extract. Outer surface protein B was tested in SDS-page.
Tested Applications
SDS-PAGE, WB
NCBI Accession Number
WP_010890379.1
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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