OspA Control protein
OspA Control protein
Product Specifications
Product Name Alternative
Outer surface protein A, Borrelia burgdorferi OspA, control protein
UniProt
P0CL66
Conjugation
Unconjugated
Source
Borrelia burgdorferi
Origin
Borrelia burgdorferi
Field of Research
Protein Biochemistry
Concentration
1 mg/ml
Dilution
ELISA: User Optimized, WB: User Optimized
Purity
OspA is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified OspA and was estimated to be greater than 90% pure.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
OspA is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 70.5 kDa for OspA-MBP, (28.1 kDa for OspA and 42.4 for MBP) in size corresponding to OspA by Western blotting in the appropriate cell lysate or extract. Outer-Surface Protein A has been tested in SDS-page and western blot.
Tested Applications
SDS-PAGE, WB
NCBI Accession Number
WP_010890378.1
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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