P35 Control protein
P35 Control protein
Product Specifications
Product Name Alternative
Bba64, Borrelia burgdorferi p35, control protein
UniProt
O50687
Conjugation
Unconjugated
Source
Borrelia burgdorferi
Origin
Borrelia burgdorferi
Field of Research
Protein Biochemistry
Concentration
1 mg/ml
Dilution
ELISA: User Optimized, WB: User Optimized
Purity
P35 is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified p35 and was estimated to be greater than 90% pure.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
P35 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 69.5 kDa for p35-MBP, (27.1 kDa for p35 and 42.4 kDa for MBP) in size corresponding to p35 by Western blotting in the appropriate cell lysate or extract. p35 kDa protein has been tested in SDS-page.
Tested Applications
SDS-PAGE, WB
NCBI Accession Number
WP_010256558.1
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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