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ErpN Control protein

ErpN Control protein

Product Specifications

Product Name Alternative

ErpN, ErpA, outer surface protein E, Borrelia burgdorferi OspE, ospE/F-Related Protein N, control protein

UniProt

Q9S060

Conjugation

Unconjugated

Source

Borrelia burgdorferi

Origin

Borrelia burgdorferi

Field of Research

Protein Biochemistry

Concentration

1 mg/mL

Dilution

ELISA: User Optimized, WB: User Optimized

Purity

ErpN/OspE is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified ErpN/OspE and was estimated to be greater than 90% pure.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

ErpN/OspE is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 59.5 kDa for OspE-MBP, (17.1 kDa for OspE and 42.4 for MBP) in size corresponding to ErpN/OspE by Western blotting in the appropriate cell lysate or extract. ErpN/OspE protein has been tested in SDS-page and western blot.

Tested Applications

SDS-PAGE, WB

NCBI Accession Number

WP_010883865.1

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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