ErpN Control protein
ErpN Control protein
Product Specifications
Product Name Alternative
ErpN, ErpA, outer surface protein E, Borrelia burgdorferi OspE, ospE/F-Related Protein N, control protein
UniProt
Q9S060
Conjugation
Unconjugated
Source
Borrelia burgdorferi
Origin
Borrelia burgdorferi
Field of Research
Protein Biochemistry
Concentration
1 mg/mL
Dilution
ELISA: User Optimized, WB: User Optimized
Purity
ErpN/OspE is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified ErpN/OspE and was estimated to be greater than 90% pure.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
ErpN/OspE is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 59.5 kDa for OspE-MBP, (17.1 kDa for OspE and 42.4 for MBP) in size corresponding to ErpN/OspE by Western blotting in the appropriate cell lysate or extract. ErpN/OspE protein has been tested in SDS-page and western blot.
Tested Applications
SDS-PAGE, WB
NCBI Accession Number
WP_010883865.1
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog