RFP protein

Recombinant RFP was synthesized with an amino terminal 6XHIS tag and was expressed in E. coli. A 3 step process was used to purify the protein: a partitioning separation using t-butanol, followed by hydrophobic interaction chromatography (HIC), and lastly IMAC nickel chromatography. Analysis by SDS-PAGE resulted in a pattern consistent with purified RFP and was estimated to be greater than 90% pure.

Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

RFP Control is suitable as a control for polyclonal or monoclonal Anti-RFP in immunological assays. The molecular weight of RFP (calculated) is 27 kDa, but in a gel it is expected to run at approximately 28-30 kDa. Recombinant RFP has been tested in SDS-page, dot blot, and western blot.