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MBP Control protein

MBP Control protein

Product Specifications

Product Name Alternative

Control protein, MBP, Maltose Binding Protein

Conjugation

Unconjugated

Concentration

5.0 mg/mL

Purity

Maltose binding protein is expressed in Escherichia coli and chromatographically purified using amylose resin.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Maltose-Binding Protein is suitable for use as a control in many assays including affinity purification, western blot and other biological assays. Optimal concentration should be determined by the researcher. Expect MW of MBP~51kDa consisting of multiple cloning sites from vector (MCS) and the MBP; molecular weight of MBP at ~40kDa. Maltose-Binding Protein has been tested in SDS-page and western blot.

Tested Applications

SDS-PAGE, WB

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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