MBP Control protein
MBP Control protein
Product Specifications
Product Name Alternative
Control protein, MBP, Maltose Binding Protein
Conjugation
Unconjugated
Concentration
5.0 mg/mL
Purity
Maltose binding protein is expressed in Escherichia coli and chromatographically purified using amylose resin.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Maltose-Binding Protein is suitable for use as a control in many assays including affinity purification, western blot and other biological assays. Optimal concentration should be determined by the researcher. Expect MW of MBP~51kDa consisting of multiple cloning sites from vector (MCS) and the MBP; molecular weight of MBP at ~40kDa. Maltose-Binding Protein has been tested in SDS-page and western blot.
Tested Applications
SDS-PAGE, WB
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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