GFP Control protein

Product Specifications

Product Name Alternative

Control protein, rGFP protein, GFP fusion protein, GFP control

UniProt

P42212

Conjugation

Unconjugated

Concentration

1.0 mg/mL

Dilution

ELISA: User Optimized, IP: User Optimized, WB: User Optimized

Purity

GFP control protein was synthesized with an amino terminal 6XHIS tag and was expressed in E. coli and purified by sequential hydrophobic interaction chromatography, DEAE chromatography and size-exclusion chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-GFP. Analysis by SDS-PAGE resulted in a pattern consistent with purified GFP and was estimated to be greater than 90% pure.

Form

Liquid (sterile filtered)

Storage Conditions

Store GFP at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

GFP Control is suitable as a control for polyclonal or monoclonal Anti-GFP in immunological assays. Researchers should determine optimal titers for applications. The molecular weight of GFP is 28 kDa. Green Fluorescent Protein has been tested in SDS-page, western blot, and immunoprecipitation.