DYKDDDDK (FLAG) peptide
DYKDDDDK (FLAG) peptide
Product Specifications
Product Name Alternative
Synthetic FLAG peptide, ddddk peptide, dykddddk peptide, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, control peptide, blocking peptide, DDK, FLAG
Conjugation
Unconjugated
Concentration
1 mg/mL
Dilution
IP: User Optimized - see PROTOCOL, WB: User Optimized as blocking peptide in PCA
Purity
Greater than 90% specific peptide by HPLC and MS.
Form
Lyophilized
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
The FLAG peptide was tested by SDS-PAGE. A working concentration of 500 μg/mL for triple FLAG peptide is typically used for competitive elution of FLAG containing proteins from anti-DYKDDDDK affinity gels. For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 μg per 1.0 μl of antibody/antiserum in per assay. See anti-DYKDDDDK FLAG affinity gel PROTOCOL for specific instructions for use. Reconstitution Buffer: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, pH 7.4. Reconstitution Volume: 1.0 mL
Tested Applications
IP, SDS-PAGE, WB
Preservative
Preservative: None. Stabilizer: None. None
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