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SLC12A2 Antibody

Goat polyclonal antibody to SLC12A2

Product Specifications

Product Name Alternative

Anti basolateral Na-K-Cl symporter antibody, anti BSC antibody, anti BSC2 antibody, anti bumetanide-sensitive sodium- (potassium) -chloride cotransporter 1 antibody, anti MGC104233 antibody, anti NKCC1 antibody, anti solute carrier family 12 (sodium/potassium/chloride transporters), member 2 antibody, anti SLC12A2 antibody

Reactivity

Human

Target

SLC12A2

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

NALQHSIRLSGVEDH

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, IHC-P: 3.75 μg/ml

Molecular Weight

131, 130

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx 75kDa band in Human, Mouse and Rat Skeletal Muscle, Heart and Brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 131kDa according to NP_001037.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Canine, Mouse, Rat

Tested Applications

ELISA, FC, IF, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

6558

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