SLC12A2 Antibody
Goat polyclonal antibody to SLC12A2
Product Specifications
Product Name Alternative
Anti basolateral Na-K-Cl symporter antibody, anti BSC antibody, anti BSC2 antibody, anti bumetanide-sensitive sodium- (potassium) -chloride cotransporter 1 antibody, anti MGC104233 antibody, anti NKCC1 antibody, anti solute carrier family 12 (sodium/potassium/chloride transporters), member 2 antibody, anti SLC12A2 antibody
Reactivity
Human
Target
SLC12A2
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
NALQHSIRLSGVEDH
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, IHC-P: 3.75 μg/ml
Molecular Weight
131, 130
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx 75kDa band in Human, Mouse and Rat Skeletal Muscle, Heart and Brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 131kDa according to NP_001037.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Canine, Mouse, Rat
Tested Applications
ELISA, FC, IF, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
6558
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